In a world where disasters and crises also evolve and cross boundaries with speed and ease, their complexity and magnitude increase, and societal repercussions often reach severe scales, it is imperative to increase citizens’ upkeep and feeling of safety and provide affected people the top-level healthcare that modern technology and current civil protection systems can offer. However, today’s emergency medical services and non-medical civil protection practitioners in a mass casualty incident scene, striving to save lives and nursing the injured, often have to rely on complicated or even outdated procedures (multiple protocols or lack of homogeneity in response methods and guidelines) and technology of the past. NIGHTINGALE will develop, integrate, test, deploy, demonstrate and validate a Novel Integrated Toolkit for Emergency Medical Response (NIT-MR) which ensures an upgrade to Prehospital life support and Triage. This will comprise a multitude of tools, services and applications required for 1) upgrading evaluation of injured and affected population and handle casualties (Triage) by offering them the means to perform digital identification, allow traceability, support fast diagnosis and prognosis and continuous monitoring and enable accurate classification of medical condition; 2) optimising pre-hospital life support and damage control through AI-based tracking, tracing, routing and utilisation enhancements of assets, resources and capacities as well as enabling continuous monitoring and correlation of vital signs and actions; 3) allowing shared response across emergency medical services, non-medical civil protection personnel, volunteers and citizens. The NIT-MR is provided at the service of the emergency medical services, non-medical civil protection personnel, volunteers and citizens for extensive testing, training and validation in the framework of a rich Training and Validation Programme of 5 Round Tables, 3 TTXs, 1 Laboratory Integration and 3 FSXs.